The cell membrane is composed mainly of a lipid bilayer with embedded proteins and carbohydrates. Using electrophoretic techniques, biosensing and spectrometric methods, we study the physicochemical properties of the plasma membrane of microorganisms and biomimicking nanoparticles. The characterization and identification of individual properties of biological membranes and their models is crucial for a better understanding of membrane structural factors.

We also develop methods to identify microorganisms in various complex matrices (e. g., biological fluids) using a combination of CE and MALDI mass spectrometry. In capillary electrophoresis, we use capillaries with internal surfaces treated with supercritical water to concentrate and purify the microbial cells being analyzed while achieving higher separation efficiencies.

For separation and purification of proteins from biological samples such as cell lysates and body fluids, we develop and use preparative instrumentation based on bidirectional isotachophoresis/moving boundary electrophoresis operating in pH gradient from 3.9 to 10.1. The protein separation and focusing take place in nonwoven fabric of trapezoidal shape and in continuous divergent flow. The liquid fractions with separated proteins are compatible with further downstream processing.