Department of Fluid Phase Separations
Department of
Fluid Phase Separations
Institute of Analytical Chemistry of the Czech Academy of SciencesInstitute of Analytical Chemistry of the CAS

Applications of compressed fluids

Our research exploits the specific characteristics of selected substances and water that they develop when subjected to elevated pressures and temperatures. This enables a reduction by an order of magnitude in the quantity of organic solvents required for the extraction of natural substances and medicines from natural sources and foodstuffs, or the complete replacement of these solvents with water.

Concerning water, the application of exceedingly high pressures and temperatures (p>250 bar, t>350°C) renders water a single medium with the capacity to cause a complete chemical decomposition of hazardous organic compounds without the generation of residual products. Alternatively, water in this state is capable of dissolving glass and silica. This is employed in the etching of glass microchips, the modification of the internal surface of fused silica capillaries, and the preparation of a novel type of chromatographic column that cannot be prepared by any other method.

Collaborations

Mendel University in Brno, Department of Crop Science, Breeding and Plant Medicine (Helena Pluháčková)
Silva Tarouca Research Institute for Landscape and Ornamental Gardening, Průhonice, Czech Republic (Marián Šinko)
St. Anne's University Hospital Brno (Filip Růžička)

Projects

GA23-04703S “Alternative route to capillary monolithic silica columns from discrete particles treated with supercritical water” (2023-2025)

R200312401 “Monitoring of biologically active compounds of selected plant species of the Asteraceae family – Matricaria chamomilla and new genotypes of the genus Echinacea” (2024)
Advanced materials development
Analysis of natural samples and foods
Instrumentation and apparatus development

Recent publications

P. Karásek, J. Planeta, M. Roth, Forming homogeneous three-dimensional structures from discrete silica microspheres using sub/supercritical water, ACS Appl. Mater. Interfaces 16 (2024) 42873-42883. B. Baranová, V. Sedlák, D. Kalemba, E. Maciejczyk, K. Wawrzyńczak, D. Gruľová, B. Kudláčková, J. Oboňa, Composition of the essential oil and aqueous leachate from Heracleum mantegazzianum seeds and their larvicidal activity against an invasive mosquito Aedes japonicus, Allelopathy J. 61 (2024) 1-16.L. Lojková, H. Pluháčková, K. Benešová, B. Kudláčková, R. Cerkal, The highest yield, or greener solvents? Latest trends in quercetin extraction methods, Trends Anal. Chem. 167 (2023) 117229. H. Pluháčková, B. Kudláčková, L. Svojanovská, M. Roth, M. Bradáčová, M. Bjelková, Effect of Field Trial on Silymarin Complex Composition and Antioxidant Assessment of Milk Thistle (Silybum marianum L. Gaertner), Plant Foods Hum. Nutr. 78 (2023) 691-697. J. Planeta, D. Moravcová, P. Karásek, M. Roth, Fabrication of monolithic capillary columns with inner diameter 50-530 μm employing a mixture of pentaerythritol tetraacrylate and polyhedral oligomeric silsesquioxane-methacrylate as crosslinkers, J. Sep. Sci. 45 (2022) 3256-3263. D. Moravcová, J. Planeta, P. Karásek, A.W.T. King, S.K. Wiedmer, Characterization and applications of a trioctyl(3/4-vinylbenzyl)phosphonium stationary phase for use in capillary liquid chromatography, J. Chromatogr. A 1666 (2022) 462866.