(Glyco)proteomic analysis

New fluorescent and MS active labels for glycan analysis are designed, synthesized and applied for analysis of glycans of various origins. The research is also focused on development of materials for oligosaccharide and glycan purification. Glycan analyses are mainly based on CE and HPLC methods with fluorescence and/or MS detection.

Collaborations

Masaryk University, Faculty of Pharmacy, Brno (Pavel Bobal)

synthesis of new labels

University of Pannonia, Veszprem, Hungary (Andras Guttman)

application of developed instrumentation for glycan analysis

Aalen University, Aalen, Germany (Christian Neusuess)

instrumentation development for glycan analysis

Projects

GA22-00236S "Development of novel fluorescent labeling tags for electrophoretic analysis of glycoproteins associated with breast cancer" (2022-2024)

9F23002 "Development of High Sensitivity and High Resolution Analytical Tools for the Brain Glycans" (2023-2026)

General scheme of designed labels

BODIPY-based labeling

Rhodamine-based labeling

Recent publications

F. Dusa, D. Smolkova, R. Cmelik, A. Guttman, J. Lavicka, Labeling of oligosaccharides and N-linked glycans by a rhodamine-based fluorescent tag for analysis by capillary electrophoresis with laser-induced fluorescence and mass spectrometry detection, Talanta 286 (2025) 127456.D. Smolkova, M. Gregus, R. Cmelik, H. Pizova, R. D. Jansen-van Vuuren, P. Bobal, J. Lavicka, Utilizing novel cationic phenylpyridine labeling in glycan profiling by capillary electrophoresis coupled to mass spectrometry, Talanta 285 (2025) 127376.D. Smolkova, D. Moravcova, J. Lavicka, Evaluation of solid-phase extraction sorbents for purification of oligosaccharides and glycans derivatized by positively charged labels followed by capillary electrophoretic analysis, J. Sep. Sci. 47 (2024) 2300705.D. Smolkova, M. Gregus, H. Vesely, R. Cmelik, H. Pizova, P. Bobal, J. Lavicka, Synthesis and application of BODIPY-based fluorescent labeling tag for oligosaccharide and N-linked glycan analysis by high-performance liquid chromatography with fluorescence detection, Anal. Chim. Acta 1285 (2024) 342032.D. Smolkova, R. Cmelik, J. Lavicka, Labeling strategies for analysis of oligosaccharides and glycans by capillary electrophoresis, Trends Anal. Chem. 163 (2023) 117068.